name:   Actinobacteriophage_2422
version:    4
date:   2019-09-14
owner:
    name:   Graham Hatfull
    email:  gfh@pitt.edu
    url:    https://www.biology.pitt.edu/person/graham-hatfull
description: >
    This database contains the 2422 manually annotated Actinobacteriophages
    analyzed in Jacobs-Sera et al. 2020.
publications:
    1:
        doi:    10.1371/journal.pone.0234636
        pmid:   32555720
        title:  >
            Genomic diversity of bacteriophages infecting Microbacterium spp
        authors:    >
            Deborah Jacobs-Sera, Lawrence A Abad, Richard M Alvey, Kirk R Anders,
            Haley G Aull, Suparna S Bhalla, Lawrence S Blumer, David W Bollivar,
            J Alfred Bonilla, Kristen A Butela, Roy J Coomans, Steven G Cresawn,
            Tom D'Elia, Arturo Diaz, Ashley M Divens, Nicholas P Edgington, Gregory
            D Frederick, Maria D Gainey, Rebecca A Garlena, Kenneth W Grant, Susan
            M R Gurney, Heather L Hendrickson, Lee E Hughes, Margaret A Kenna, Karen
            K Klyczek, Hari Kotturi, Travis N Mavrich, Angela L McKinney, Evan C
            Merkhofer, Sally D Molloy, Denise L Monti, Dana A Pape-Zambito, Richard
            S Pollenz, Welkin H Pope, Nathan S Reyna, Claire A Rinehart, Dan A Russell,
            Christopher D Shaffer, Viknesh Sivanathan, Ty H Stoner, Joseph Stukey,
            C Nicole Sunnen, Sara S Tolsma, Philippos K Tsourkas, Jamie R Wallen,
            Vassie C Ware, Marcie H Warner, Jacqueline M Washington, Kristi M Westover,
            JoAnn L Whitefleet-Smith, Helen I Wiersma-Koch, Daniel C Williams, Kira
            M Zack, Graham F Hatfull
        abstract:   >
            The bacteriophage population is vast, dynamic, old, and genetically diverse.
            The genomics of phages that infect bacterial hosts in the phylum Actinobacteria
            show them to not only be diverse but also pervasively mosaic, and replete with
            genes of unknown function. To further explore this broad group of bacteriophages,
            we describe here the isolation and genomic characterization of 116 phages that
            infect Microbacterium spp. Most of the phages are lytic, and can be grouped into
            twelve clusters according to their overall relatedness; seven of the phages are
            singletons with no close relatives. Genome sizes vary from 17.3 kbp to 97.7 kbp,
            and their G+C% content ranges from 51.4% to 71.4%, compared to ~67% for their
            Microbacterium hosts. The phages were isolated on five different Microbacterium
            species, but typically do not efficiently infect strains beyond the one on which
            they were isolated. These Microbacterium phages contain many novel features,
            including very large viral genes (13.5 kbp) and unusual fusions of structural
            proteins, including a fusion of VIP2 toxin and a MuF-like protein into a single
            gene. These phages and their genetic components such as integration systems,
            recombineering tools, and phage-mediated delivery systems, will be useful resources
            for advancing Microbacterium genetics.
resources: ~