name:   Actinobacteriophage_685
version:    3
date:   2016-07-19
owner:
    name:   Graham Hatfull
    email:  gfh@pitt.edu
    url:    https://www.biology.pitt.edu/person/graham-hatfull
description: >
    This database contains the 685 manually annotated Actinobacteriophages
    analyzed in Cheng et al. 2018.
publications:
    1:
        doi:    10.1186/s12866-018-1159-y
        pmid:   29490612
        title:  >
            Complete genomic sequences of Propionibacterium freudenreichii phages
            from Swiss cheese reveal greater diversity than Cutibacterium (formerly
            Propionibacterium) acnes phages
        authors:    >
            Lucy Cheng, Laura J Marinelli, Noël Grosset, Sorel T Fitz-Gibbon, Charles
            A Bowman, Brian Q Dang, Daniel A Russell, Deborah Jacobs-Sera, Baochen Shi,
            Matteo Pellegrini, Jeff F Miller, Michel Gautier, Graham F Hatfull,
            Robert L Modlin
        abstract:   >
            A remarkable exception to the large genetic diversity often observed for
            bacteriophages infecting a specific bacterial host was found for the
            Cutibacterium acnes (formerly Propionibacterium acnes) phages, which are
            highly homogeneous. Phages infecting the related species, which is also a
            member of the Propionibacteriaceae family, Propionibacterium freudenreichii,
            a bacterium used in production of Swiss-type cheeses, have also been
            described and are common contaminants of the cheese manufacturing process.
            However, little is known about their genetic composition and diversity.
            We obtained seven independently isolated bacteriophages that infect P.
            freudenreichii from Swiss-type cheese samples, and determined their
            complete genome sequences. These data revealed that all seven phage isolates
            are of similar genomic length and GC% content, but their genomes are highly
            diverse, including genes encoding the capsid, tape measure, and tail
            proteins. In contrast to C. Acnes phages, all P. Freudenreichii phage genomes
            encode a putative integrase protein, suggesting they are capable of lysogenic
            growth. This is supported by the finding of related prophages in some P.
            freudenreichii strains. The seven phages could further be distinguished as
            belonging to two distinct genomic types, or 'clusters', based on
            nucleotide sequences, and host range analyses conducted on a collection of
            P. freudenreichii strains show a higher degree of host specificity than is
            observed for the C. acnes phages.
            Overall, our data demonstrate P. freudenreichii bacteriophages are distinct
            from C. acnes phages, as evidenced by their higher genetic diversity,
            potential for lysogenic growth, and more restricted host ranges. This
            suggests substantial differences in the evolution of these related species
            from the Propionibacteriaceae family and their phages, which is potentially
            related to their distinct environmental niches.
resources: ~