name:   Actinobacteriophage_706
version:    1
date:   2016-02-25
owner:
    name:   Graham Hatfull
    email:  gfh@pitt.edu
    url:    https://www.biology.pitt.edu/person/graham-hatfull
description: >
    This database contains the 706 manually annotated Actinobacteriophages
    analyzed in Dedrick et al 2016.
publications:
    1:
        doi:    10.1111/mmi.13414
        pmid:   27146086
        title:  >
            Function, expression, specificity, diversity, and incompatibility of
            actinobacteriophage parABS systems
        authors:    >
            Rebekah M Dedrick, Travis N Mavrich, Wei L Ng, Juan C Cervantes Reyes,
            Matthew R Olm, Rachael E Rush, Deborah Jacobs-Sera, Daniel A Russel,
            Graham F Hatfull
        abstract:   >
            More than 180 individual phages infecting hosts in the phylum Actinobacteria
            have been sequenced and grouped into Cluster A because of their similar overall
            nucleotide sequences and genome architectures. These Cluster A phages are either
            temperate or derivatives of temperate parents, and most have an integration cassette
            near the center of the genome containing an integrase gene and attP. However, about
            20% of the phages lack an integration cassette, which is replaced by a 1.4 kbp
            segment with predicted partitioning functions, including plasmid-like parA and parB
            genes. Phage RedRock forms stable lysogens in Mycobacterium smegmatis in which the
            prophage replicates at 2.4 copies/chromosome and the partitioning system confers
            prophage maintenance. The parAB genes are expressed upon RedRock infection of M.
            smegmatis, but are down-regulated once lysogeny is established by binding of RedRock
            ParB to parS-L, one of two centromere-like sites flanking the parAB genes. The RedRock
            parS-L and parS-R sites are composed of eight directly repeated copies of an 8 bp motif
            that is recognized by ParB. The actinobacteriophage parABS cassettes span considerable
            sequence diversity and specificity, providing a suite of tools for use in mycobacterial
            genetics.
resources: ~