name:   Actinobacteriophage_937
version:    1
date:   2017-04-07
owner:
    name:   Graham Hatfull
    email:  gfh@pitt.edu
    url:    https://www.biology.pitt.edu/person/graham-hatfull
description: >
    This database contains the 937 manually annotated Actinobacteriophages analyzed
    in Dedrick et al. 2017.
publications:
    1:
        doi:    10.1186/s12866-017-1131-2
        pmid:   29197343
        title:  >
            Expression and evolutionary patterns of mycobacteriophage D29 and its
            temperate close relatives
        authors:    >
            Rebekah M Dedrick, Travis N Mavrich, Wei L Ng, Graham F Hatfull
        abstract:   >
            Mycobacteriophages are viruses that infect Mycobacterium hosts. A large
            collection of phages known to infect the same bacterial host strain -
            Mycobacterium smegmatis mc2155 - exhibit substantial diversity and
            characteristically mosaic architectures. The well-studied lytic
            mycobacteriophage D29 appears to be a deletion derivative of a putative
            temparate parent, although its parent has yet to be identified.
            Here we describe three newly-isolated temperate phages - Kerberos, Pomar16,
            and StarStuff - that are related to D29, and are predicted to be very close
            relatives of its putative temperate parent, revealing the repressor and
            additional genes that are lost in D29. Transcriptional profiles show the
            patterns of both lysogenic and lytic gene expression and identify
            highly-expressed, abundant, stable, small non-coding transcripts made
            from the Pleft early lytic promoter, and which are toxic to M. smegmatis.
            Comparative genomics of phages D29, Kerberos, Pomar16, and StarStuff
            provide insights into bacteriophage evolution, and comparative
            transcriptomics identifies the pattern of lysogenic and lytic expression
            with unusual features including highly expressed, small, non-coding RNAs.
resources: ~