name:   McProf_DB
version:    1
date:   2020-08-24
owner:
    name:   Sally Molloy
    email:  sally.dixon@maine.edu
    url:    https://umaine.edu/biomed/home/faculty/sally-molloy/
description:    >
    This database is derived from the Actino_Draft database, and includes the Mycobacterium
    prophage McProf.  It was created to facilitate analyses performed in Molloy et al. 2021.
publications:
    1:
        doi:    10.1186/s12866-021-02224-z
        pmid:   34107872
        title:  >
            Increased whiB7 expression and antibiotic resistance in Mycobacterium chelonae
            carrying two prophage
        authors:    >
            Jaycee Cushman, Emma Freeman, Sarah McCallister, Anna Schumann, Keith W Hutchison,
            Sally D. Molloy
        abstract:   >
            Background: The global rise in the incidence of non-tuberculosis mycobacterial 
            infections is of increasing concern due their high levels of intrinsic antibiotic 
            resistance. Although integrated viral genomes, called prophage, are linked to 
            increased antibiotic resistance in some bacterial species, we know little of their 
            role in mycobacterial drug resistance.

            Results: We present here for the first time, evidence of increased antibiotic 
            resistance and expression of intrinsic antibiotic resistance genes in a strain of 
            Mycobacterium chelonae carrying prophage. Strains carrying the prophage McProf 
            demonstrated increased resistance to amikacin. Resistance in these strains was 
            further enhanced by exposure to sub-inhibitory concentrations of the antibiotic, 
            acivicin, or by the presence of a second prophage, BPs. Increased expression of 
            the virulence gene, whiB7, was observed in strains carrying both prophages, 
            BPs and McProf, relative to strains carrying a single prophage or no prophages.

            Conclusions: This study provides evidence that prophage alter expression of important 
            mycobacterial intrinsic antibiotic resistance genes and additionally offers insight 
            into the role prophage may play in mycobacterial adaptation to stress.
resources:  ~